Shythdf3
WebFigure Legend Snippet: a , b mCherry-GFP-LC3 was transfected into shNS and shYTHDF3 MEFs, and autophagosome (yellow) and autolysosome (red) formation was examined. Scale bar, 20 μm. c , d Endogenous ULK1, ATG13, ATG14, and DFCP1 puncta in shNS and shYTHDF3 MEFs were immunostained after nutrient deficiency, visualized with confocal … WebFeb 1, 2024 · N 6-methyladenosine (m 6 A) is a reversible modification in mRNA and has been shown to regulate processing, translation and decay of mRNA. However, the roles of m 6 A modification in neuronal development are still not known. Here, we found that the m 6 A eraser FTO is enriched in axons and can be locally translated. Axon-specific inhibition of …
Shythdf3
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WebOct 4, 2024 · Scale bar, 20 μm. e Histograms of R/GFIR of 3000 events analyzed by flow cytometry after AO staining in shYTHDF3 MEFs and shNS MEFs, with or without 20 nM … WebGAPDH is used as a loading control. d Immunoblot analyses of YTHDF3 restored (shYTHDF3 + YTHDF3) and control (shYTHDF3 + Con) MEFs following nutrient starvation …
WebMDA-MB-231 shYTHDF3 1: Sample type: RNA : Source name: MDA-MB-231 shYTHDF3: Organism: Homo sapiens: Characteristics: cell line: MDA-MB-231Br cell type: Breast Cancer Cell Line genotype/variation: YTHDF3 knockdown: Treatment protocol: MDA-MB231Br cells were transduced with shControl and shYTHDF3 lentivirus and selected by puromycin. WebJun 28, 2024 · Scale bar, 10 μm (n=3 independent experiments); c. Quantification of YTHDF3 protein expression by immuno-staining in shScram and shYTHDF3 cells (Scram, sh1, and sh2).
WebDec 14, 2024 · To identify molecules that are differentially expressed in brain metastases of breast cancer, we analyzed Gene Expression Omnibus public datasets for gene expression differences between human breast cancer brain metastases and primary breast tumors (Schulten et al., 2024), and between human breast cancer MDA-MB-231 parental cells and … WebMar 7, 2024 · Abstract. N 6-Methyladenosine (m 6 A) is a dynamic mRNA modification which regulates protein expression in various posttranscriptional levels. Functional studies of m …
WebOct 29, 2024 · Brain metastasis is a major cause of cancer mortality, but its molecular mechanisms are severely understudied. In addition, little is known regarding the role of m 6 A reader YTHDF3 in human diseases. Here we show that YTHDF3 overexpression clinically correlates with brain metastases in breast cancer patients.
WebTo rule out the possibility that Ythdf3 is compensated for during the embryonic stage of Ythdf3-/-mice, we generated two efficient short hairpin RNA (shRNA) against Ythdf3: … high glucose and altWeb5-GGACGTTCCCAATAGCCAACT-3; shYthdf3, 5-GCACCTAAACCAACTTCTTGG-3; shLacZ (con-trol),5-GCATAAACCCGCCACTCATCT-3.The lentiviral overexpression vector pLVX- IRES-eGFP was made from pLVX-IRES-Hyg by replacing HygR with eGFP. The lentiviral particles were generated using a second generation packaging system. In brief, HEK293T highglow jewelers garden city miWebOct 7, 2024 · The objectives are to identify expression profiles that are specific to shYTHDF3 breast cancer cell to identify new the signaling pathway of YTHDF3 for brain metastasis … howi know if password dieWebMar 10, 2024 · g, k Cell proliferation measured by CCK8 assay of HTR8/SVneo cells stably expressing shYTHDF3 (g) and YTHDF3 (k) as indicated. The value of OD 450 was … how i know lyrics lil durkWebDec 14, 2024 · To identify molecules that are differentially expressed in brain metastases of breast cancer, we analyzed Gene Expression Omnibus public datasets for gene … high glucose and wound healingWebMay 1, 2024 · Animals and generation of Ythdf1 cKO mice. For generation of Ythdf1 conditional knockout (cKO) mice, exon 4 of mouse Ythdf1 gene was targeted with the consideration that exon 4 encodes the YTD domain. A loxP site and an FRT-flanked neomycin resistance gene (Neo r) coupled with a loxP site were inserted in intron 3 and … how i know if i have covidWebFigure Legend Snippet: a , b mCherry-GFP-LC3 was transfected into shNS and shYTHDF3 MEFs, and autophagosome (yellow) and autolysosome (red) formation was examined. Scale bar, 20 μm. c , d Endogenous ULK1, ATG13, ATG14, and DFCP1 puncta in shNS and shYTHDF3 MEFs were immunostained after nutrient deficiency, visualized with confocal … high glucose and pregnancy effects