Mhat3f cells
Webb22 jan. 1999 · MhAT3F is a well differentiated mouse hepatocyte-like cell line ( 26, 32) that has a protein secretion profile very similar to primary hepatocytes. Cells were pretreated for 15 min with the MTP inhibitor prior to pulse labeling with l - [ 35 S]methionine for 30 min. After a 120-min chase, secreted apoB were immunoprecipitated. Webb19 dec. 2007 · The mhAT3F undergo a cycle of contraction and respreading under the influence of TGF-β. Cells were grown on plastic dishes, treated with TGF-β1 (5 ng/ml), and observed by time-lapse microscopy for 96 h. Representative images of a low-power field and threefold enlargement of one cluster (indicated by an arrow in the top panel).
Mhat3f cells
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Webbhexokinase activity is present in mhAT3F cells. This might explain why the effects of glucose on gene expres-sion were not maximal at a glucose concentration of 5 … WebbAcute liver failure (ALF) is associated with massive hepatocyte cell death and high mortality rates. Therapeutic approaches targeting hepatocyte injury in ALF are hampered by the activation of distinct stimulus-dependent pathways, mechanism of cell death and a limited therapeutic window.
Webb2 okt. 1998 · When mhAT3F cells were cultured for 24 h in the presence of increasing glucose concentrations (0, 5, and 17 mmol/liter), an accumulation of Glut2mRNA was … Webb15 juni 2005 · The mhAT3F hepatocyte cell line, established from a mouse with liver-targeted production of SV40 large T antigen, retains a pattern of gene expression …
WebbWhen exposed to TGF-beta, the mhAT3F cells, immortalized, highly differentiated hepatocytes, maintained their epithelial morphology and underwent dramatic alterations of adhesion, leading to partial or complete detachment from a culture plate, followed by readhesion and spreading. WebbCell Culture Human hepatoblastoma (HepG2) cells were routinely maintained in Williams’s medium (Eurobio, France) supplemented with 10% fetal calf serum and 1% streptomycin at 37°C. Only confluent cells were used in the study. The well-differentiated mhAT3F hepatoma cell line was derived from the tumorous liver of a transgenic mouse
Webb23 okt. 2003 · In mhAT3F hepatoma cells, GFP-karyopherin α2 accumulated massively in the cytoplasm within minutes of glucose extracellular addition and returned to the …
WebbAmong them is the hepatocyte nuclear factor 3 (HNF3) family, composed of the HNF3 alpha, HNF3 beta, and HNF3 gamma proteins, which are expressed in the liver and have very similar fork head DNA binding domains. The regulatory regions of numerous hepatocyte-specific genes contain HNF3 binding sites. github ndis filterWebbIn mhAT3F hepatoma cells, GFP-karyopherin a2 accumu-lated massively in the cytoplasm within minutes of glu-cose extracellular addition and returned to the nucleus after … fuquay performing artsWebbThe microphthalmia-associated transcription factor (Mitf) is critical for mast cell development based on the severe mast cell deficiency seen in Mitf mutant mice. Mitf … github ndarrayWebb31 dec. 1996 · The glucose-induced GLUT2 mRNA accumulation was decreased by dibutyryl-cAMP both in mhAT3F cells and in primary hepatocytes. A putative cAMP-responsive element (CRE) is localized at the -1074/-1068 bp region of the promoter. github nds romsWebb1 jan. 1994 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy. github ncursesWebbmhAT3F cells MIN6 cells HEK293 cells Not stated Pullen et al. miRNA-939 SLC34A2 Gastric cancer cell lines and tissue 5-Fluorouacil Zhang et al. miRNA-369-3p SLC35F5 NSCLC cells ... fuquay varina arts councilWebbMorphology of NIH3T3 cells during exposure to GF-AFC reagent. Panel A shows a field of cells photographed immediately after additional of the GF-AFC reagent. Panel B shows … fuquay varina homes for rent