Web9 mei 2024 · Genomic DNA was isolated from cryopreserved peripheral blood or bone marrow mononuclear cells and subjected to targeted, amplicon-based, next-generation sequencing of coding regions of MDS-associated genes, using Ion Torrent (National Cancer Institute of Canada/Canadian Cancer Trials Group Alliance and Nationwide samples; n = … Web14 jul. 2024 · Libraries were purified using Ampure XP beads before quantitation by qPCR with the Ion Library TaqMan™ quantitation Kit (Cat: 4468802; Thermo Fisher Scientific, UK). Libraries were normalised to 70pM before pooling and templating onto an Ion 540™ Chip (Cat: A22765, Thermo Fisher Scientific, UK) using an Ion Chef™ (Cat: 4484177, …
Ion Library TaqMan™ Quantitation Kit Ion Torrent™ 4468802 - 和 …
Web9 feb. 2024 · One hundred nanograms of genomic DNA was used in library preparation with Ion AmpliSeq Exome RDY Kit 1×8 (Cat. No. A38262); then, the libraries were purified using a magnetic plate and subsequently quantified using Ion Library TaqMan quantitation kit (Part number 4468802) and Real-time PCR instrument 7500 Fast (Life Technologies, … WebIon 文库 TaqMan 定量试剂盒可使 Ion 片段文库中的可扩增模板定量用于 Ion Torrent 测序工作流程中的模板制备。. 该试剂盒包括设计用于 Ion 文库接头和 Ion 文库对照品的定制 … great principles of computing阅读报告
Identification of a SCN4A The Author(s) 2024 mutation in a large ...
WebSample barcoding was performed using Ion Express Barcode Adapter 1–16 kit. After ligating the adapters, the libraries were purified using CleanPCR (Clean NA, GC Biotech, The Netherlands). Each library was then quantified using Ion Library TaqMan Quantitation Kit (#4468802, ThermoFisher Scientific, USA). Web3 apr. 2024 · Ion Library TaqMan™ Quantitation Kit (Thermo, 4468802). 3 Methods This protocol begins with gDNA extraction from blood collected in PAXgene DNA tubes. However, other collection methods are acceptable. The protocol is geared to manual NGS library preparation using AmpliSeq TM technology and sequencing on the Ion Proton … WebIon Library TaqMan® Quantitation Kit Product No. 4468802 Lot No. 1712056 Date of Manufacture21DEC2024 TEST SPECIFICATION RESULT STANDARD DEVIATION … great principles of computing作者