Biochemical characterization of rice glutelin

WebNov 2, 2008 · A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the chromosomal glutelin … WebJun 1, 2003 · L. Q. Qu 81-298-38-8373 81-298-38-8397 [email protected] X. L. Wei H. Satoh T. Kumamaru M. Ogawa F. Takaiwa Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan …

Characterization of rice glutelin fibrils and their effect on

National Center for Biotechnology Information WebJun 1, 1985 · The two major subunits of rice glutelin, the acidic (alpha) and basic (beta) polypeptides were purified by chromatofocusing and cation … earth renewable products https://doddnation.com

Characterization of a new rice glutelin gene - Oxford Academic

WebREP-1 was further purified to a single polypeptide of 36 kDa. REP-1 digested in vitro both the acidic and basic subunits of rice glutelin, the major seed storage protein of rice. Determination of the N-terminal amino acid sequence and experiments with protease inhibitors indicated that REP-1 is a cysteine endopeptidase. WebDec 31, 2024 · Enhancing the solubility of rice glutelin in neutral aqueous solution is the prerequisite for the development of rice protein drinks and ingredients. Herein, glutelin … WebJun 26, 2007 · The overall electrophoretic profile and alpha glutelin subunits in different rice samples ranged from 12.1 to 100.9 and 32.1 to 34.0 kDa, respectively, while beta glutelin subunits ranged from 23. ... cto eindhoven

Electrophoretic and Functional Mapping of Indica Rice Glutelin …

Category:Solubility and conformational characterization of rice glutelin after

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Biochemical characterization of rice glutelin

Unique Glutelin Expression Patterns and Seed Endosperm Structure ... - Rice

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Biochemical characterization of rice glutelin

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WebRice Glutelins SpringerLink Seed Proteins Cite as Rice Glutelins Fumio Takaiwa, Masahiro Ogawa & Thomas W. Okita Chapter Circuit is open Abstract Despite being the major … WebSep 1, 2024 · Rice glutelin has high asparagine and glutamine contents (Liu et al., 2011). Thus, it likely has high ability to form fibril aggregates, since Tang et al. (2010) …

WebThe nucleotide sequence revealed that the iso-electric point variation of the pI6.50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable … WebNov 2, 2008 · Abstract. A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the …

WebDec 1, 2024 · A spectrofluorometric method was utilized to provide additional information about the interaction of rice glutelin and gallic acid. Fluorescence excitation was carried out at 280 nm, since this wavelength is sensitive to changes in the local environments of tryptophan and tyrosine groups, which provides indirect information about alterations in … WebThe nucleotide sequence revealed that the iso-electric point variation of the pI6.50/α-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable …

WebSep 1, 2024 · Here we investigated and characterized the formation of thermal-induced rice glutelin fibrils at 85 °C and pH 2.0. The potential effects of rice glutelin fibrils on the in vitro starch digestibility were also evaluated. Thioflavin T fluorescence spectral analysis revealed extensive formation of rice glutelin fibrils after 4 h of incubation at pH 2.0 and 85 °C.

WebThe rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin α-2 subunit while the α-1 subunit increased (α-1H/α-2L), was used in this study. Two-dimensional electrophoresis … ct oems renewalWebINTRODUCTION. The major endospermic rice protein is glutelin,[1, 2] which comprised up to 70 to 80% of total rice protein.Janick also found 70% of glutelin protein in rice flour as compared to other cereals, which were higher in prolamin contents. The biochemical characterization of rice proteins shows the important nutritional properties and these … ct oems pcrWebSep 1, 2012 · The Osborne method comprising of a 2-step in vitro digestion of rice flour was used to extract albumin, globulin, prolamin, and glutelin by using pepsin and trypsin. Subsequently, the antioxidant … Expand earth removerWebJul 5, 2024 · Background Rice is not only an essential food but also a source of high quality protein. Polyploidy is an evolutionary trajectory in plants, and enhancing glutelin by polyploidization is an attractive strategy for improving the nutritional value of rice seeds and presents a great potential for enhancing the commercial value of rice. Elucidating the … earth renewable resourcesWebApr 1, 2002 · Very little is known about the gene that regulates the biochemical and cellular processes responsible for the formation of PBs. Rice mutants that show variations in the profile patterns of storage proteins have been identified and assigned into four classes (Kumamaru et al., 1988; Ogawa et al., 1989). earth renewable energy co. ltdWebNov 2, 2008 · Rice glutelin originates from the same ancestral gene as 11S globulin. Rice glutelin is synthesized as a M r 57 000 (57 kDa) precursor protein and is transported … earth renewable technologies cnpjWebChickpeas are the third most abundant legume crop worldwide, having a high protein content (14.9–24.6%) with interesting technological properties, thus representing a sustainable alternative to animal proteins. In this study, the surface and structural properties of total (TE) and sequential (ALB, GLO, and GLU) protein fractions isolated from defatted … earth renewable technologies curitiba